Stable Isotope-Labeled Lipidomics to Unravel the Heterogeneous Development Lipotoxicity

Normalization Standard Consisting of 1,000 Stable Isotope Labeled Peptides

Stable Isotope Labeled n-Alkanes to Assess Digesta Passage Kinetics through the Digestive Tract of Ruminants

We describe the use of carbon stable isotope ((13)C) labeled n-alkanes as a potential internal tracer to assess passage kinetics of ingested nutrients in ruminants. Plant cuticular n-alkanes originating from intrinsically (13)C labeled ryegrass plants were pulse dosed intraruminally in four rumen-cannulated lactating dairy cows receiving four contrasting ryegrass silage treatments that differed...

Stable Isotope Application in Animal Nutrition Science

The application of stable isotope analysis (SIA) has become a standard scientific approach in Agricultural and Ecological researches and, more in general, in several disciplines such as biology, botany, zoology, organic chemistry, climatology, and nutrition. The main objectives of this paper are (1) to provide a simple definition of stable isotopes and (2) to illustrate analytical measurement m...

Formation of hydroxymethyl DNA adducts in rats orally exposed to stable isotope labeled methanol.

Methanol is a large volume industrial chemical and widely used solvent and fuel additive. Methanol's well known toxicity and use in a wide spectrum of applications has raised long-standing environmental issues over its safety, including its carcinogenicity. Methanol has not been listed as a carcinogen by any regulatory agency; however, there are debates about its carcinogenic potential. Formald...

Lipidomics in biomarker development

Research into the cell biology of intracellular pathogens has provided information on host-pathogen interactions and on the importance of lipids at various stages in the interactions. Lipids are “gatekeepers” in important chemical reactions involving cell signaling during pathogen docking, invasion and traffic in and out of cells. Working with the Novartis Institute for Tropical Diseases, our t...